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digitonin  (Gold Biotechnology Inc)


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    Structured Review

    Gold Biotechnology Inc digitonin
    Digitonin, supplied by Gold Biotechnology Inc, used in various techniques. Bioz Stars score: 94/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/digitonin+special+grade/Digitonin+Special+Grade/bio_rxiv__64898__2026__03__29__713301-219-13-14
    Average 94 stars, based on 34 article reviews
    digitonin - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Microscopy:

    Article Title: Recessive pathogenic variants in MCAT cause combined oxidative phosphorylation deficiency
    Article Snippet: say (Pierce), and used in assays described below. BN-PAGE was performed using the Invitrogen NativePAGE system. One hundred micrograms of mitochondria were resuspended in 1× pink lysis buffer (Invitrogen, BN20032). Digitonin (GoldBio D-180-2.5) was added to a final concentration of 1% mass/volume. Samples were incubated on ice for 15 min, then spun for 20 min at 20,000 × g . Six microliters of NativePAGE sample buffer (Invitrogen, BN20041) was add

    Protease Inhibitor:

    Article Title: Recessive pathogenic variants in MCAT cause combined oxidative phosphorylation deficiency
    Article Snippet: say (Pierce), and used in assays described below. BN-PAGE was performed using the Invitrogen NativePAGE system. One hundred micrograms of mitochondria were resuspended in 1× pink lysis buffer (Invitrogen, BN20032). Digitonin (GoldBio D-180-2.5) was added to a final concentration of 1% mass/volume. Samples were incubated on ice for 15 min, then spun for 20 min at 20,000 × g . Six microliters of NativePAGE sample buffer (Invitrogen, BN20041) was add

    Concentration Assay:

    Article Title: Recessive pathogenic variants in MCAT cause combined oxidative phosphorylation deficiency
    Article Snippet: say (Pierce), and used in assays described below. BN-PAGE was performed using the Invitrogen NativePAGE system. One hundred micrograms of mitochondria were resuspended in 1× pink lysis buffer (Invitrogen, BN20032). Digitonin (GoldBio D-180-2.5) was added to a final concentration of 1% mass/volume. Samples were incubated on ice for 15 min, then spun for 20 min at 20,000 × g . Six microliters of NativePAGE sample buffer (Invitrogen, BN20041) was add

    Fractionation:

    Article Title: Recessive pathogenic variants in MCAT cause combined oxidative phosphorylation deficiency
    Article Snippet: say (Pierce), and used in assays described below. BN-PAGE was performed using the Invitrogen NativePAGE system. One hundred micrograms of mitochondria were resuspended in 1× pink lysis buffer (Invitrogen, BN20032). Digitonin (GoldBio D-180-2.5) was added to a final concentration of 1% mass/volume. Samples were incubated on ice for 15 min, then spun for 20 min at 20,000 × g . Six microliters of NativePAGE sample buffer (Invitrogen, BN20041) was add

    SYBR Green Assay:

    Article Title: Recessive pathogenic variants in MCAT cause combined oxidative phosphorylation deficiency
    Article Snippet: say (Pierce), and used in assays described below. BN-PAGE was performed using the Invitrogen NativePAGE system. One hundred micrograms of mitochondria were resuspended in 1× pink lysis buffer (Invitrogen, BN20032). Digitonin (GoldBio D-180-2.5) was added to a final concentration of 1% mass/volume. Samples were incubated on ice for 15 min, then spun for 20 min at 20,000 × g . Six microliters of NativePAGE sample buffer (Invitrogen, BN20041) was add

    Transfection:

    Article Title: Recessive pathogenic variants in MCAT cause combined oxidative phosphorylation deficiency
    Article Snippet: say (Pierce), and used in assays described below. BN-PAGE was performed using the Invitrogen NativePAGE system. One hundred micrograms of mitochondria were resuspended in 1× pink lysis buffer (Invitrogen, BN20032). Digitonin (GoldBio D-180-2.5) was added to a final concentration of 1% mass/volume. Samples were incubated on ice for 15 min, then spun for 20 min at 20,000 × g . Six microliters of NativePAGE sample buffer (Invitrogen, BN20041) was add

    Software:

    Article Title: Recessive pathogenic variants in MCAT cause combined oxidative phosphorylation deficiency
    Article Snippet: say (Pierce), and used in assays described below. BN-PAGE was performed using the Invitrogen NativePAGE system. One hundred micrograms of mitochondria were resuspended in 1× pink lysis buffer (Invitrogen, BN20032). Digitonin (GoldBio D-180-2.5) was added to a final concentration of 1% mass/volume. Samples were incubated on ice for 15 min, then spun for 20 min at 20,000 × g . Six microliters of NativePAGE sample buffer (Invitrogen, BN20041) was add

    Polyacrylamide Gel Electrophoresis:

    Article Title: Recessive pathogenic variants in MCAT cause combined oxidative phosphorylation deficiency
    Article Snippet: say (Pierce), and used in assays described below. BN-PAGE was performed using the Invitrogen NativePAGE system. One hundred micrograms of mitochondria were resuspended in 1× pink lysis buffer (Invitrogen, BN20032). Digitonin (GoldBio D-180-2.5) was added to a final concentration of 1% mass/volume. Samples were incubated on ice for 15 min, then spun for 20 min at 20,000 × g . Six microliters of NativePAGE sample buffer (Invitrogen, BN20041) was add

    Plasmid Preparation:

    Article Title: Recessive pathogenic variants in MCAT cause combined oxidative phosphorylation deficiency
    Article Snippet: say (Pierce), and used in assays described below. BN-PAGE was performed using the Invitrogen NativePAGE system. One hundred micrograms of mitochondria were resuspended in 1× pink lysis buffer (Invitrogen, BN20032). Digitonin (GoldBio D-180-2.5) was added to a final concentration of 1% mass/volume. Samples were incubated on ice for 15 min, then spun for 20 min at 20,000 × g . Six microliters of NativePAGE sample buffer (Invitrogen, BN20041) was add

    Lysis:

    Article Title: Recessive pathogenic variants in MCAT cause combined oxidative phosphorylation deficiency
    Article Snippet: say (Pierce), and used in assays described below. BN-PAGE was performed using the Invitrogen NativePAGE system. One hundred micrograms of mitochondria were resuspended in 1× pink lysis buffer (Invitrogen, BN20032). Digitonin (GoldBio D-180-2.5) was added to a final concentration of 1% mass/volume. Samples were incubated on ice for 15 min, then spun for 20 min at 20,000 × g . Six microliters of NativePAGE sample buffer (Invitrogen, BN20041) was add

    Respiration Assay:

    Article Title: Recessive pathogenic variants in MCAT cause combined oxidative phosphorylation deficiency
    Article Snippet: say (Pierce), and used in assays described below. BN-PAGE was performed using the Invitrogen NativePAGE system. One hundred micrograms of mitochondria were resuspended in 1× pink lysis buffer (Invitrogen, BN20032). Digitonin (GoldBio D-180-2.5) was added to a final concentration of 1% mass/volume. Samples were incubated on ice for 15 min, then spun for 20 min at 20,000 × g . Six microliters of NativePAGE sample buffer (Invitrogen, BN20041) was add

    Incubation:

    Article Title: Recessive pathogenic variants in MCAT cause combined oxidative phosphorylation deficiency
    Article Snippet: say (Pierce), and used in assays described below. BN-PAGE was performed using the Invitrogen NativePAGE system. One hundred micrograms of mitochondria were resuspended in 1× pink lysis buffer (Invitrogen, BN20032). Digitonin (GoldBio D-180-2.5) was added to a final concentration of 1% mass/volume. Samples were incubated on ice for 15 min, then spun for 20 min at 20,000 × g . Six microliters of NativePAGE sample buffer (Invitrogen, BN20041) was add

    Western Blot:

    Article Title: Recessive pathogenic variants in MCAT cause combined oxidative phosphorylation deficiency
    Article Snippet: say (Pierce), and used in assays described below. BN-PAGE was performed using the Invitrogen NativePAGE system. One hundred micrograms of mitochondria were resuspended in 1× pink lysis buffer (Invitrogen, BN20032). Digitonin (GoldBio D-180-2.5) was added to a final concentration of 1% mass/volume. Samples were incubated on ice for 15 min, then spun for 20 min at 20,000 × g . Six microliters of NativePAGE sample buffer (Invitrogen, BN20041) was add

    Colorimetric Assay:

    Article Title: Recessive pathogenic variants in MCAT cause combined oxidative phosphorylation deficiency
    Article Snippet: say (Pierce), and used in assays described below. BN-PAGE was performed using the Invitrogen NativePAGE system. One hundred micrograms of mitochondria were resuspended in 1× pink lysis buffer (Invitrogen, BN20032). Digitonin (GoldBio D-180-2.5) was added to a final concentration of 1% mass/volume. Samples were incubated on ice for 15 min, then spun for 20 min at 20,000 × g . Six microliters of NativePAGE sample buffer (Invitrogen, BN20041) was add

    Centrifugation:

    Article Title: Recessive pathogenic variants in MCAT cause combined oxidative phosphorylation deficiency
    Article Snippet: say (Pierce), and used in assays described below. BN-PAGE was performed using the Invitrogen NativePAGE system. One hundred micrograms of mitochondria were resuspended in 1× pink lysis buffer (Invitrogen, BN20032). Digitonin (GoldBio D-180-2.5) was added to a final concentration of 1% mass/volume. Samples were incubated on ice for 15 min, then spun for 20 min at 20,000 × g . Six microliters of NativePAGE sample buffer (Invitrogen, BN20041) was add

    Blue Native PAGE:

    Article Title: Recessive pathogenic variants in MCAT cause combined oxidative phosphorylation deficiency
    Article Snippet: say (Pierce), and used in assays described below. BN-PAGE was performed using the Invitrogen NativePAGE system. One hundred micrograms of mitochondria were resuspended in 1× pink lysis buffer (Invitrogen, BN20032). Digitonin (GoldBio D-180-2.5) was added to a final concentration of 1% mass/volume. Samples were incubated on ice for 15 min, then spun for 20 min at 20,000 × g . Six microliters of NativePAGE sample buffer (Invitrogen, BN20041) was add

    Expressing:

    Article Title: Recessive pathogenic variants in MCAT cause combined oxidative phosphorylation deficiency
    Article Snippet: say (Pierce), and used in assays described below. BN-PAGE was performed using the Invitrogen NativePAGE system. One hundred micrograms of mitochondria were resuspended in 1× pink lysis buffer (Invitrogen, BN20032). Digitonin (GoldBio D-180-2.5) was added to a final concentration of 1% mass/volume. Samples were incubated on ice for 15 min, then spun for 20 min at 20,000 × g . Six microliters of NativePAGE sample buffer (Invitrogen, BN20041) was add

    BIA-KA:

    Article Title: Recessive pathogenic variants in MCAT cause combined oxidative phosphorylation deficiency
    Article Snippet: say (Pierce), and used in assays described below. BN-PAGE was performed using the Invitrogen NativePAGE system. One hundred micrograms of mitochondria were resuspended in 1× pink lysis buffer (Invitrogen, BN20032). Digitonin (GoldBio D-180-2.5) was added to a final concentration of 1% mass/volume. Samples were incubated on ice for 15 min, then spun for 20 min at 20,000 × g . Six microliters of NativePAGE sample buffer (Invitrogen, BN20041) was add

    Isolation:

    Article Title: Recessive pathogenic variants in MCAT cause combined oxidative phosphorylation deficiency
    Article Snippet: say (Pierce), and used in assays described below. BN-PAGE was performed using the Invitrogen NativePAGE system. One hundred micrograms of mitochondria were resuspended in 1× pink lysis buffer (Invitrogen, BN20032). Digitonin (GoldBio D-180-2.5) was added to a final concentration of 1% mass/volume. Samples were incubated on ice for 15 min, then spun for 20 min at 20,000 × g . Six microliters of NativePAGE sample buffer (Invitrogen, BN20041) was add

    Membrane:

    Article Title: Recessive pathogenic variants in MCAT cause combined oxidative phosphorylation deficiency
    Article Snippet: say (Pierce), and used in assays described below. BN-PAGE was performed using the Invitrogen NativePAGE system. One hundred micrograms of mitochondria were resuspended in 1× pink lysis buffer (Invitrogen, BN20032). Digitonin (GoldBio D-180-2.5) was added to a final concentration of 1% mass/volume. Samples were incubated on ice for 15 min, then spun for 20 min at 20,000 × g . Six microliters of NativePAGE sample buffer (Invitrogen, BN20041) was add

    Staining:

    Article Title: Recessive pathogenic variants in MCAT cause combined oxidative phosphorylation deficiency
    Article Snippet: say (Pierce), and used in assays described below. BN-PAGE was performed using the Invitrogen NativePAGE system. One hundred micrograms of mitochondria were resuspended in 1× pink lysis buffer (Invitrogen, BN20032). Digitonin (GoldBio D-180-2.5) was added to a final concentration of 1% mass/volume. Samples were incubated on ice for 15 min, then spun for 20 min at 20,000 × g . Six microliters of NativePAGE sample buffer (Invitrogen, BN20041) was add

    Flow Cytometry:

    Article Title: Recessive pathogenic variants in MCAT cause combined oxidative phosphorylation deficiency
    Article Snippet: say (Pierce), and used in assays described below. BN-PAGE was performed using the Invitrogen NativePAGE system. One hundred micrograms of mitochondria were resuspended in 1× pink lysis buffer (Invitrogen, BN20032). Digitonin (GoldBio D-180-2.5) was added to a final concentration of 1% mass/volume. Samples were incubated on ice for 15 min, then spun for 20 min at 20,000 × g . Six microliters of NativePAGE sample buffer (Invitrogen, BN20041) was add

    Bicinchoninic Acid Protein Assay:

    Article Title: Recessive pathogenic variants in MCAT cause combined oxidative phosphorylation deficiency
    Article Snippet: say (Pierce), and used in assays described below. BN-PAGE was performed using the Invitrogen NativePAGE system. One hundred micrograms of mitochondria were resuspended in 1× pink lysis buffer (Invitrogen, BN20032). Digitonin (GoldBio D-180-2.5) was added to a final concentration of 1% mass/volume. Samples were incubated on ice for 15 min, then spun for 20 min at 20,000 × g . Six microliters of NativePAGE sample buffer (Invitrogen, BN20041) was add

    Sequencing:

    Article Title: Recessive pathogenic variants in MCAT cause combined oxidative phosphorylation deficiency
    Article Snippet: say (Pierce), and used in assays described below. BN-PAGE was performed using the Invitrogen NativePAGE system. One hundred micrograms of mitochondria were resuspended in 1× pink lysis buffer (Invitrogen, BN20032). Digitonin (GoldBio D-180-2.5) was added to a final concentration of 1% mass/volume. Samples were incubated on ice for 15 min, then spun for 20 min at 20,000 × g . Six microliters of NativePAGE sample buffer (Invitrogen, BN20041) was add

    Solvent:

    Article Title: Recessive pathogenic variants in MCAT cause combined oxidative phosphorylation deficiency
    Article Snippet: say (Pierce), and used in assays described below. BN-PAGE was performed using the Invitrogen NativePAGE system. One hundred micrograms of mitochondria were resuspended in 1× pink lysis buffer (Invitrogen, BN20032). Digitonin (GoldBio D-180-2.5) was added to a final concentration of 1% mass/volume. Samples were incubated on ice for 15 min, then spun for 20 min at 20,000 × g . Six microliters of NativePAGE sample buffer (Invitrogen, BN20041) was add

    Agarose Gel Electrophoresis:

    Article Title: Recessive pathogenic variants in MCAT cause combined oxidative phosphorylation deficiency
    Article Snippet: say (Pierce), and used in assays described below. BN-PAGE was performed using the Invitrogen NativePAGE system. One hundred micrograms of mitochondria were resuspended in 1× pink lysis buffer (Invitrogen, BN20032). Digitonin (GoldBio D-180-2.5) was added to a final concentration of 1% mass/volume. Samples were incubated on ice for 15 min, then spun for 20 min at 20,000 × g . Six microliters of NativePAGE sample buffer (Invitrogen, BN20041) was add

    Generated:

    Article Title: Recessive pathogenic variants in MCAT cause combined oxidative phosphorylation deficiency
    Article Snippet: say (Pierce), and used in assays described below. BN-PAGE was performed using the Invitrogen NativePAGE system. One hundred micrograms of mitochondria were resuspended in 1× pink lysis buffer (Invitrogen, BN20032). Digitonin (GoldBio D-180-2.5) was added to a final concentration of 1% mass/volume. Samples were incubated on ice for 15 min, then spun for 20 min at 20,000 × g . Six microliters of NativePAGE sample buffer (Invitrogen, BN20041) was add

    Glo Assay:

    Article Title: Recessive pathogenic variants in MCAT cause combined oxidative phosphorylation deficiency
    Article Snippet: say (Pierce), and used in assays described below. BN-PAGE was performed using the Invitrogen NativePAGE system. One hundred micrograms of mitochondria were resuspended in 1× pink lysis buffer (Invitrogen, BN20032). Digitonin (GoldBio D-180-2.5) was added to a final concentration of 1% mass/volume. Samples were incubated on ice for 15 min, then spun for 20 min at 20,000 × g . Six microliters of NativePAGE sample buffer (Invitrogen, BN20041) was add

    Blocking Assay:

    Article Title: Recessive pathogenic variants in MCAT cause combined oxidative phosphorylation deficiency
    Article Snippet: say (Pierce), and used in assays described below. BN-PAGE was performed using the Invitrogen NativePAGE system. One hundred micrograms of mitochondria were resuspended in 1× pink lysis buffer (Invitrogen, BN20032). Digitonin (GoldBio D-180-2.5) was added to a final concentration of 1% mass/volume. Samples were incubated on ice for 15 min, then spun for 20 min at 20,000 × g . Six microliters of NativePAGE sample buffer (Invitrogen, BN20041) was add

    Recombinant:

    Article Title: Recessive pathogenic variants in MCAT cause combined oxidative phosphorylation deficiency
    Article Snippet: say (Pierce), and used in assays described below. BN-PAGE was performed using the Invitrogen NativePAGE system. One hundred micrograms of mitochondria were resuspended in 1× pink lysis buffer (Invitrogen, BN20032). Digitonin (GoldBio D-180-2.5) was added to a final concentration of 1% mass/volume. Samples were incubated on ice for 15 min, then spun for 20 min at 20,000 × g . Six microliters of NativePAGE sample buffer (Invitrogen, BN20041) was add

    Mutagenesis:

    Article Title: Recessive pathogenic variants in MCAT cause combined oxidative phosphorylation deficiency
    Article Snippet: say (Pierce), and used in assays described below. BN-PAGE was performed using the Invitrogen NativePAGE system. One hundred micrograms of mitochondria were resuspended in 1× pink lysis buffer (Invitrogen, BN20032). Digitonin (GoldBio D-180-2.5) was added to a final concentration of 1% mass/volume. Samples were incubated on ice for 15 min, then spun for 20 min at 20,000 × g . Six microliters of NativePAGE sample buffer (Invitrogen, BN20041) was add

    RNA Sequencing:

    Article Title: Recessive pathogenic variants in MCAT cause combined oxidative phosphorylation deficiency
    Article Snippet: say (Pierce), and used in assays described below. BN-PAGE was performed using the Invitrogen NativePAGE system. One hundred micrograms of mitochondria were resuspended in 1× pink lysis buffer (Invitrogen, BN20032). Digitonin (GoldBio D-180-2.5) was added to a final concentration of 1% mass/volume. Samples were incubated on ice for 15 min, then spun for 20 min at 20,000 × g . Six microliters of NativePAGE sample buffer (Invitrogen, BN20041) was add

    Virus:

    Article Title: Recessive pathogenic variants in MCAT cause combined oxidative phosphorylation deficiency
    Article Snippet: say (Pierce), and used in assays described below. BN-PAGE was performed using the Invitrogen NativePAGE system. One hundred micrograms of mitochondria were resuspended in 1× pink lysis buffer (Invitrogen, BN20032). Digitonin (GoldBio D-180-2.5) was added to a final concentration of 1% mass/volume. Samples were incubated on ice for 15 min, then spun for 20 min at 20,000 × g . Six microliters of NativePAGE sample buffer (Invitrogen, BN20041) was add

    Clone Assay:

    Article Title: Recessive pathogenic variants in MCAT cause combined oxidative phosphorylation deficiency
    Article Snippet: say (Pierce), and used in assays described below. BN-PAGE was performed using the Invitrogen NativePAGE system. One hundred micrograms of mitochondria were resuspended in 1× pink lysis buffer (Invitrogen, BN20032). Digitonin (GoldBio D-180-2.5) was added to a final concentration of 1% mass/volume. Samples were incubated on ice for 15 min, then spun for 20 min at 20,000 × g . Six microliters of NativePAGE sample buffer (Invitrogen, BN20041) was add

    Extraction:

    Article Title: Recessive pathogenic variants in MCAT cause combined oxidative phosphorylation deficiency
    Article Snippet: say (Pierce), and used in assays described below. BN-PAGE was performed using the Invitrogen NativePAGE system. One hundred micrograms of mitochondria were resuspended in 1× pink lysis buffer (Invitrogen, BN20032). Digitonin (GoldBio D-180-2.5) was added to a final concentration of 1% mass/volume. Samples were incubated on ice for 15 min, then spun for 20 min at 20,000 × g . Six microliters of NativePAGE sample buffer (Invitrogen, BN20041) was add

    Clarification Assay:

    Article Title: Recessive pathogenic variants in MCAT cause combined oxidative phosphorylation deficiency
    Article Snippet: say (Pierce), and used in assays described below. BN-PAGE was performed using the Invitrogen NativePAGE system. One hundred micrograms of mitochondria were resuspended in 1× pink lysis buffer (Invitrogen, BN20032). Digitonin (GoldBio D-180-2.5) was added to a final concentration of 1% mass/volume. Samples were incubated on ice for 15 min, then spun for 20 min at 20,000 × g . Six microliters of NativePAGE sample buffer (Invitrogen, BN20041) was add

    Clinical Proteomics:

    Article Title: Recessive pathogenic variants in MCAT cause combined oxidative phosphorylation deficiency
    Article Snippet: say (Pierce), and used in assays described below. BN-PAGE was performed using the Invitrogen NativePAGE system. One hundred micrograms of mitochondria were resuspended in 1× pink lysis buffer (Invitrogen, BN20032). Digitonin (GoldBio D-180-2.5) was added to a final concentration of 1% mass/volume. Samples were incubated on ice for 15 min, then spun for 20 min at 20,000 × g . Six microliters of NativePAGE sample buffer (Invitrogen, BN20041) was add

    Magnetic Beads:

    Article Title: Recessive pathogenic variants in MCAT cause combined oxidative phosphorylation deficiency
    Article Snippet: say (Pierce), and used in assays described below. BN-PAGE was performed using the Invitrogen NativePAGE system. One hundred micrograms of mitochondria were resuspended in 1× pink lysis buffer (Invitrogen, BN20032). Digitonin (GoldBio D-180-2.5) was added to a final concentration of 1% mass/volume. Samples were incubated on ice for 15 min, then spun for 20 min at 20,000 × g . Six microliters of NativePAGE sample buffer (Invitrogen, BN20041) was add

    MTT Assay:

    Article Title: Recessive pathogenic variants in MCAT cause combined oxidative phosphorylation deficiency
    Article Snippet: say (Pierce), and used in assays described below. BN-PAGE was performed using the Invitrogen NativePAGE system. One hundred micrograms of mitochondria were resuspended in 1× pink lysis buffer (Invitrogen, BN20032). Digitonin (GoldBio D-180-2.5) was added to a final concentration of 1% mass/volume. Samples were incubated on ice for 15 min, then spun for 20 min at 20,000 × g . Six microliters of NativePAGE sample buffer (Invitrogen, BN20041) was add

    Construct:

    Article Title: Recessive pathogenic variants in MCAT cause combined oxidative phosphorylation deficiency
    Article Snippet: say (Pierce), and used in assays described below. BN-PAGE was performed using the Invitrogen NativePAGE system. One hundred micrograms of mitochondria were resuspended in 1× pink lysis buffer (Invitrogen, BN20032). Digitonin (GoldBio D-180-2.5) was added to a final concentration of 1% mass/volume. Samples were incubated on ice for 15 min, then spun for 20 min at 20,000 × g . Six microliters of NativePAGE sample buffer (Invitrogen, BN20041) was add

    Multiple Displacement Amplification:

    Article Title: Recessive pathogenic variants in MCAT cause combined oxidative phosphorylation deficiency
    Article Snippet: say (Pierce), and used in assays described below. BN-PAGE was performed using the Invitrogen NativePAGE system. One hundred micrograms of mitochondria were resuspended in 1× pink lysis buffer (Invitrogen, BN20032). Digitonin (GoldBio D-180-2.5) was added to a final concentration of 1% mass/volume. Samples were incubated on ice for 15 min, then spun for 20 min at 20,000 × g . Six microliters of NativePAGE sample buffer (Invitrogen, BN20041) was add

    Clear Native PAGE:

    Article Title: Recessive pathogenic variants in MCAT cause combined oxidative phosphorylation deficiency
    Article Snippet: say (Pierce), and used in assays described below. BN-PAGE was performed using the Invitrogen NativePAGE system. One hundred micrograms of mitochondria were resuspended in 1× pink lysis buffer (Invitrogen, BN20032). Digitonin (GoldBio D-180-2.5) was added to a final concentration of 1% mass/volume. Samples were incubated on ice for 15 min, then spun for 20 min at 20,000 × g . Six microliters of NativePAGE sample buffer (Invitrogen, BN20041) was add

    CRISPR:

    Article Title: Recessive pathogenic variants in MCAT cause combined oxidative phosphorylation deficiency
    Article Snippet: say (Pierce), and used in assays described below. BN-PAGE was performed using the Invitrogen NativePAGE system. One hundred micrograms of mitochondria were resuspended in 1× pink lysis buffer (Invitrogen, BN20032). Digitonin (GoldBio D-180-2.5) was added to a final concentration of 1% mass/volume. Samples were incubated on ice for 15 min, then spun for 20 min at 20,000 × g . Six microliters of NativePAGE sample buffer (Invitrogen, BN20041) was add

    Synthesized:

    Article Title: Recessive pathogenic variants in MCAT cause combined oxidative phosphorylation deficiency
    Article Snippet: say (Pierce), and used in assays described below. BN-PAGE was performed using the Invitrogen NativePAGE system. One hundred micrograms of mitochondria were resuspended in 1× pink lysis buffer (Invitrogen, BN20032). Digitonin (GoldBio D-180-2.5) was added to a final concentration of 1% mass/volume. Samples were incubated on ice for 15 min, then spun for 20 min at 20,000 × g . Six microliters of NativePAGE sample buffer (Invitrogen, BN20041) was add

    Bradford Assay:

    Article Title: Recessive pathogenic variants in MCAT cause combined oxidative phosphorylation deficiency
    Article Snippet: say (Pierce), and used in assays described below. BN-PAGE was performed using the Invitrogen NativePAGE system. One hundred micrograms of mitochondria were resuspended in 1× pink lysis buffer (Invitrogen, BN20032). Digitonin (GoldBio D-180-2.5) was added to a final concentration of 1% mass/volume. Samples were incubated on ice for 15 min, then spun for 20 min at 20,000 × g . Six microliters of NativePAGE sample buffer (Invitrogen, BN20041) was add

    Cell Culture:

    Article Title: Recessive pathogenic variants in MCAT cause combined oxidative phosphorylation deficiency
    Article Snippet: say (Pierce), and used in assays described below. BN-PAGE was performed using the Invitrogen NativePAGE system. One hundred micrograms of mitochondria were resuspended in 1× pink lysis buffer (Invitrogen, BN20032). Digitonin (GoldBio D-180-2.5) was added to a final concentration of 1% mass/volume. Samples were incubated on ice for 15 min, then spun for 20 min at 20,000 × g . Six microliters of NativePAGE sample buffer (Invitrogen, BN20041) was add

    Real-time Polymerase Chain Reaction:

    Article Title: Recessive pathogenic variants in MCAT cause combined oxidative phosphorylation deficiency
    Article Snippet: say (Pierce), and used in assays described below. BN-PAGE was performed using the Invitrogen NativePAGE system. One hundred micrograms of mitochondria were resuspended in 1× pink lysis buffer (Invitrogen, BN20032). Digitonin (GoldBio D-180-2.5) was added to a final concentration of 1% mass/volume. Samples were incubated on ice for 15 min, then spun for 20 min at 20,000 × g . Six microliters of NativePAGE sample buffer (Invitrogen, BN20041) was add

    Protein Concentration:

    Article Title: Recessive pathogenic variants in MCAT cause combined oxidative phosphorylation deficiency
    Article Snippet: say (Pierce), and used in assays described below. BN-PAGE was performed using the Invitrogen NativePAGE system. One hundred micrograms of mitochondria were resuspended in 1× pink lysis buffer (Invitrogen, BN20032). Digitonin (GoldBio D-180-2.5) was added to a final concentration of 1% mass/volume. Samples were incubated on ice for 15 min, then spun for 20 min at 20,000 × g . Six microliters of NativePAGE sample buffer (Invitrogen, BN20041) was add

    Reverse Transcription:

    Article Title: Recessive pathogenic variants in MCAT cause combined oxidative phosphorylation deficiency
    Article Snippet: say (Pierce), and used in assays described below. BN-PAGE was performed using the Invitrogen NativePAGE system. One hundred micrograms of mitochondria were resuspended in 1× pink lysis buffer (Invitrogen, BN20032). Digitonin (GoldBio D-180-2.5) was added to a final concentration of 1% mass/volume. Samples were incubated on ice for 15 min, then spun for 20 min at 20,000 × g . Six microliters of NativePAGE sample buffer (Invitrogen, BN20041) was add

    Imaging:

    Article Title: Recessive pathogenic variants in MCAT cause combined oxidative phosphorylation deficiency
    Article Snippet: say (Pierce), and used in assays described below. BN-PAGE was performed using the Invitrogen NativePAGE system. One hundred micrograms of mitochondria were resuspended in 1× pink lysis buffer (Invitrogen, BN20032). Digitonin (GoldBio D-180-2.5) was added to a final concentration of 1% mass/volume. Samples were incubated on ice for 15 min, then spun for 20 min at 20,000 × g . Six microliters of NativePAGE sample buffer (Invitrogen, BN20041) was add

    RNA Sequencing Assay:

    Article Title: Recessive pathogenic variants in MCAT cause combined oxidative phosphorylation deficiency
    Article Snippet: say (Pierce), and used in assays described below. BN-PAGE was performed using the Invitrogen NativePAGE system. One hundred micrograms of mitochondria were resuspended in 1× pink lysis buffer (Invitrogen, BN20032). Digitonin (GoldBio D-180-2.5) was added to a final concentration of 1% mass/volume. Samples were incubated on ice for 15 min, then spun for 20 min at 20,000 × g . Six microliters of NativePAGE sample buffer (Invitrogen, BN20041) was add

    Viability Assay:

    Article Title: Recessive pathogenic variants in MCAT cause combined oxidative phosphorylation deficiency
    Article Snippet: say (Pierce), and used in assays described below. BN-PAGE was performed using the Invitrogen NativePAGE system. One hundred micrograms of mitochondria were resuspended in 1× pink lysis buffer (Invitrogen, BN20032). Digitonin (GoldBio D-180-2.5) was added to a final concentration of 1% mass/volume. Samples were incubated on ice for 15 min, then spun for 20 min at 20,000 × g . Six microliters of NativePAGE sample buffer (Invitrogen, BN20041) was add

    Sterility:

    Article Title: Recessive pathogenic variants in MCAT cause combined oxidative phosphorylation deficiency
    Article Snippet: say (Pierce), and used in assays described below. BN-PAGE was performed using the Invitrogen NativePAGE system. One hundred micrograms of mitochondria were resuspended in 1× pink lysis buffer (Invitrogen, BN20032). Digitonin (GoldBio D-180-2.5) was added to a final concentration of 1% mass/volume. Samples were incubated on ice for 15 min, then spun for 20 min at 20,000 × g . Six microliters of NativePAGE sample buffer (Invitrogen, BN20041) was add

    Labeling:

    Article Title: Recessive pathogenic variants in MCAT cause combined oxidative phosphorylation deficiency
    Article Snippet: say (Pierce), and used in assays described below. BN-PAGE was performed using the Invitrogen NativePAGE system. One hundred micrograms of mitochondria were resuspended in 1× pink lysis buffer (Invitrogen, BN20032). Digitonin (GoldBio D-180-2.5) was added to a final concentration of 1% mass/volume. Samples were incubated on ice for 15 min, then spun for 20 min at 20,000 × g . Six microliters of NativePAGE sample buffer (Invitrogen, BN20041) was add



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